Studies of the Prothrombin Activation Pathway Utilizing Radioimmunoassays for the F 2 / F
نویسنده
چکیده
We have evaluated the efficacy of utilizing radioimmunoassays (RIAs) for prothrombin activation fragments (F2/F1 +2) and for thrombin-antithrombin complex (TAT) in purified systems and in whole blood. During venipuncture. appropriate anticoagulants were employed in order to prevent the generation of thrombin and factor Xa. The RIAs were shown to be specific for F2/F1 +2 as well as TAT and did not interact with other plasma components. Initially. thrombin generation was studied in a purified human system of prothrombin. antithrombin. factor Xa. and factor V as well as phospholipid and Ca . Under these conditions. the kinetics of F2/F1 +2 and TAT generation were virtually superimposable. However. when factor V was omitted from the reaction mixture. a significantly greater amount of F2/F142 as compared to TAT was observable. Subsequently. prothrombin activation was monitored during the
منابع مشابه
Studies of the prothrombin activation pathway utilizing radioimmunoassays for the F2/F1 + 2 fragment and thrombin--antithrombin complex.
We have evaluated the efficacy of utilizing radioimmunoassays (RIAs) for prothrombin activation fragments (F2/F1 + 2) and for thrombin--antithrombin complex (TAT) in purified systems and in whole blood. During venipuncture, appropriate anticoagulants were employed in order to prevent the generation of thrombin and factor Xa. The RIAs were shown to be specific for F2/F1 + 2 as well as TAT and di...
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